A group of tropical disease caused by a range of parasitic roundworms and larvae that transmit disease to humans. About 200 million people are affected by filariasis, which occurs in tropic and subtropics areas of Southeast Asia, South America, Africa, and the Pacific, Mosquitoes inject the worm larvae when they bite, which migrate to the lymph nodes where they develop into mature worms in about a year.
Some of the species live in the lymphatic vessels and block them, causing ELEPHANTIASIS (swelling of limbs with thickened, coarse skin). Another type of worm can be seen and felt just underneath the skin, which produces irritating and painful swellings called calabar swellings.
Bancroftian filariasis (Wuchereria bancrofti filarial nematode) is spread widely throughout Africa, southern and southeastern Asia, the Pacific, and the tropical and subtropical regions of South America.
Malayan filariasis (Brugia malayi filarial nematode) is found only in southern and southeastern Asia. Imported States, especially among immigrants from the Caribbean and Pacific islands.
Bancroftian and Malayan filariasis are transmitted to humans by the bite of an infected mosquito. The infective larvae that are transmitted into humans via the bite pass into the human lymph system, where they develop to maturity during a six to 12 month period. Fertilized mature female worms release embryos that develop into moving larvae (microfilariae). Which appear in the human blood system at night only
Causes of filariasis
Lymphatic filariasis is caused by threadlike worms (nematodes) that live in the lymphatic system; it is transmitted by the bite of a mosquito carrying infective larvae. The larvae develop into adult worms in the lymphatic vessels, causing severe damage and swelling (lymphodema).
The most common filarial species is Wuchereria bancrofti, but Brugia malayi and Brugia timori cause the infection in Asia. It takes several mosquito bites over many months to cause the infection. People living in tropical and subtropical settings are at the greatest risk for infection.
Cutaneous filariasis caused by Loa loa is transmitted by the mango fly or deer fly; onchocerca transmits microfilariae via the black fly; and Manosonella streptocerca transmits infection via a midge. Dirofilaria immitis may cause lesions in the lung periphery. Depending on the species, incubation may be 3 to 12 months.
Symptoms of filariasis
Lymphatic filariasis is otherwise called Elephantiasis. Here the patient presents with swollen genitals and a swollen scrotum. Patients have difficulty in mobility and prefer to sit on their enlarged scrotum.
In addition, the backflow of lymphatic fluid causes the legs to enlarge and the leakage of the lymphatic fluid causes the verrucous and thick appearance of the skin mimicking elephant legs.
Other symptoms are testicular and inguinal pain, slight fever, exfoliation of the affected skin and inguinal lymphadeonpathies. In subcutaneous filariasis, the symptoms are limited to the skin and the eye. The skin manifests with erythematous, pruritis, edematous, with scab like eruptions.
The skin thickness, described as hanging groin and leopard skin. The eye manifestations are corneal fibrosis, keratitis, choroiditis, glaucoma, iridocyclitis and optic atrophy.
All of these eye findings can cause blindness. Patients with serous cavity filariasis are usually asymptomatic. If ever they have symptoms, they are mild fever, pruritus and mild abdominal pain.
Diagnosis of filariasis
The adult worms residing in the lymphatics are inaccessible, so diagnosis is based on finding microfilariae in the peripheral blood. The level of filaria is inversely related to the clinical signs because much of the damage is due to immunological responses to the microfilariae rather than to the organisms themselves.
Furthermore the presence of microfilariae does not necessarily mean that they are causing clinical problems and conversely a lack of microfilariae in the blood does not exclude a diagnosis of filariasis. The peripheral blood finding must therefore be assessed in the context of the clinical picture.
To optimize the chances of findings scanty microfilariae in the blood, the sample should be taken at the appropriate time for the expected peak concentration of microfilariae (i.e. around midnight or midday for nocturnally and diurnally periodic forms respectively).
There are many techniques for demonstrating microfilariae in the laboratory. The simplest method is a wet preparation of fresh blood. Microfilariae will survive in venous blood collected into EDTA for 2 days at room temperature. Motile microfilariae can be seen on a slide under low power and can be counted in a counting chamber. Numbers of microfilariae may be low, so concentration techniques are often required.
Sensitivity can be increased by passing the whole blood sample through a 3 µm Nuclepore filter membrane and then stating the microfilariae trapped in the filter.
For species identification, thick and thin blood films should be stained with Giemsa or haematoxylin and the microfilariae differential according to the pattern of their sheaths, nuclei distributions and size. The edges of the film should be examined carefully as microfilariae tend to be concentrated at the periphery and are easily missed if the microscopist goes straight onto high power in the center of film.
Detection of circulating antigen by enzyme linked immunosorbent assay (ELISA) or ICT has replaced microscopy for the diagnosis of bancroftian, but not brugian, filariasis.
An antigen immunochromatography card test is available for the detection of W. bancrofti, which does not react with other filariae and is highly sensitive (100%) and specific (92%).
Filarial DNA can be detected by PCR, and ultrasound can help to identify adult worms within the lymphatic system. Serological tests are not very helpful in the diagnosis as most individuals from endemic areas have antibodies to crude filarial antigens and there is cross reactivity with other filariae and nematodes.
Complications of filariasis
Filarial infection can cause personal and socioeconomic hardships due to its debilitating consequences. Meningoencephalopathy and localized necrotizing granulomas are severe complications of L. loa infection. D. immitis may cause pulmonary infarction if the larvae lodge in the branches of the pulmonary arteries. Lymphatic filariasis may result in Hydroceles or elephantiasis of the extremities or genitals. A secondary bacterial infection may cause elephantiasis, blindness, and infection of the skin and lymphatic system
Treatment of filariasis