| 1 Departamento de Biologia, Universidade Estadual
de Maringá, Av Colombo, 5790, 87020, Maringá, Paraná,
Brasil.
ABSTRACT: The aim of this work was to analyze
the effect of the homeopathic solution Sulphur on some radish growth
variables. Five dynamizations (potencies) of Sulphur were used (5
CH,12 CH, 30 CH, 200 CH and 1 MCH) on a centesimal scale. Treatments
were carried out in vases outdoors and the homeopathic solution
was applied weekly. The fresh and dry matter mass of the shoot and
the root system, the average length of the root system, the average
plant length, the length of the largest leaf and the root diameter
were analyzed. The application of Sulphur improved the general conditions
of the plants in practically all the studied variables, when compared
with a control. The dynamizations 5 CH, 12 CH, 30 CH and 1 MCH showed
better responses. The control and the dynamization 200 CH were the
treatments that presented the most negative results. The results
suggest that the homeopathic Sulphur can be an alternative to improve
the productivity and appearance of commercial agricultural products,
with substantial reduction in agricultural input.
Key words: dynamization (potentization), Raphanus sativus L.
Introduction
The basic principle of homeopathy, according to Samuel Hahnemann
(1755 to 1843), is the use of dynamized (potentized) solutions prepared
from animal, vegetable or mineral substances, or sick tissues. For
homeopathy, the disease or the unbalance is seen as an energetic
unbalance and not as the action of the virus, bacteria and others
(Bonato, 2002). The homeopathic solution promotes the re-establishment
of the plants through improvement of their natural defenses. The
use of homeopathy in agriculture was made official in 1999 (Brasil,
1999). Now homeopathic science is being used efficiently in the
control of plagues (Fazolin et al., 2000), diseases (Verma et al.,
1969; Kumar, 1980; Khanna e Chandra, 1983), to increase the active
principles in medicinal plants (Carvalho 2001; Castro, 2002), plant
detoxification for metals as aluminium (Rocha et al., 2002; Moretti
et al., 2002) and copper (Almeida, 2002), increasing plant growth
rate and productivity (Castro, 2002). This suggested the idea of
verifying the applicability of the homeopathic solution Sulphur
in some variables of the radish, for growth and productivity.
Material and methods
The experiment was conducted in the Didactic and Experimental Garden
of Departamento de Biologia da Universidade Estadual de Maringá,
from September 3 to October 25, 2002.
Obtaining the homeopathic solutions
The main Sulphur solutions were obtained on a centesimal scale
from a suitable pharmaceutical laboratory (Farmácia Homeopática
João Vicente Martins- Maringá -PR).
Application of the homeopathic solution
The treatments consisted of applications of 5 homeopathic dynamizations
of Sulphur 5 CH, 12 CH, 30 CH, 200 CH and 1 MCH) and water used
as control. The Sulphur was applied in the proportion of twenty
drops (1.5mL) per water liter. Each vase received 100mL of the solution
every seven days.
Performance of the experiment
Radish seeds were sown directly in 3-liter vases, receiving, as
substratum, soil, decomposed bovine manure and sand in the proportion
of 3:2:1, respectively. Ferralsols originating from the Didactic
and Experimental Garden were used.
After one week, the plants were thinned, leaving just two plants
per vase. Soluble fertilizers were not used during the experiment.
The data were collected for 31 days. The experiment was carried
out according to double-blind methodology, i.e. the experimenter
and the applicator ignored the dynamization of the solution that
was being applied.
Statistics
The experimental delineation was entirely in randomized blocks,
with 4 repetitions. The treatments consisted of 5 Sulphur dynamizations
on a Hahnemannian centesimal scale, in addition to the control.
In the qualitative tests, the averages were compared using the Scott-Knott
test at 5% probability.
As regards the quantitative variables, the models were chosen based
on the significance of the regression coefficients using the “t”
test at 5% probability.
Obtaining the data
Data collection was made every 7 days. Evaluations were always
made in the same afternoon period.
Quantified variables
Length of the largest leaf
Evaluation of leaf length was made weekly. The measures were taken
starting from the beginning of the petiole to the leaf apex.
Plant height
Determination of plant height in each phase was made measuring
the distance between the base of the stem and maximum plant height.
Fresh matter of the shoot and the root system
The plants collected on the 31st day after being sown had their
shoots and root system highlighted, with the aid of scissors, and
immediately weighed on an analytic scale.
Dry matter of the shoot and the root system
After the fresh matter of the shoot and the root system was weighed,
the material was placed in a kiln (70°C) at constant mass and
then weighed on an analytic scale.
Results and discussion
Effect of Sulphur on leaf length
Average leaf length was influenced by the application of the homeopathic
solution Sulphur
(Figure 1). The dynamizations 5 CH, 12 CH, 30 CH and 1 MCH showed
longer average leaf length in relation to dynamization 200 CH and
to the control (water). It was observed that the plants responded
differently when several dynamizations of the same solution were
applied. In several situations, the homeopathic solution also inhibits
leaf growth (Castro, 2002). It should be emphasized that, starting
from the dynamization 12 CH, according to the “Avogadro´s
Number” (6.02 x 1023), physical molecules of the solution
do not exist, only energy (Schembri, 1992).

Effect of Sulphur on plant height
Plant height was influenced by different dynamizations of the homeopathic
solution, except 7 and 14 days after sowing (Table 1).
Three weeks after sowing, it was verified that the treatments 5
CH, 12 CH, 30 CH and 1 MCH presented height significantly higher
than the control
Effect of the homeopathic solution Sulphur 261 and the potency
200 CH. This behavior was practically identical to that observed
for leaf length (Figure 1). The same tendency occurred for plant
height at 21 and 28 days and in the general average (Table 1). It
was observed that the growth alterations reflected the alternation
of responses to the dynamizations. Alteration due to increasing
dynamizations has been verified in many experiments (Davenas et
al., 1988). In homeopathic science, it is common to observe the
same solution causing very different effects in accordance with
the dynamization, sometimes stimulating a lot, other times stimulating
very little. This has been observed in clinical practice from the
time of Hahnemann (Godoy, 1988; Castro, 2002). Fazolin et al. (2000),
for example, verified this when carrying out an experiment seeking
the control of Cerotoma tingomarianus in beans. The homeopathic
solution Ignatia decreased the consumption of bean leaves by Cerotoma
tingomarianus in the decimal dynamizations D5 and D15 and increased
consumption in the dynamizations D9 and D29. The same phenomenon
was observed using silver nitrate in the dynamizations D24 and D26,
which increased the growth of wheat seedlings, while the intermediary
dynamization D25 depressed the growth of the seedlings (Pongratz
et al., 1998). This phenomena is still not explained by science,
but it is believed to be related to existing rhythmic movements
in nature, and also to the law of similitude that occurs between
the applied homeopathic solution and the organism that receives
it (Vithoulkas, 1980; Godoy, 1988).

According to the regression equations in Figure 2, the homeopathy
Sulphur demonstrated much higher angular coefficient values for
the dynamizations 5CH, 12 CH, 30 CH and 1 MCH than for the control
and the dynamization 200 CH.
This means that all the dynamizations (except for 200 CH) determined
increase in the growth rate of the radish plants and suggests, in
this case, that the homeopathy Sulphur interferes positively, accelerating
plant metabolism. This fact was also observed by Castro (2002) in
the application of Phosphorus in beets.

Effect of Sulphur on root diameter
The application of the homeopathy Sulphur in the dynamizations
12 CH, 30 CH and 1 MCH increased the average diameter of the radish
roots significantly in relation to the control and the dynamizations
200 CH and 5 CH (Figure 3). The behaviour presented here was similar
to that observed for the first described items. The average root
diameter of the plants treated with 12 CH, 30 CH and 1 MCH was approximately
3 times greater than the control. This result is of great importance,
because the applied homeopathy resulted in larger roots, leading
to higher productivity and higher commercial value of the product.
Again, it was observed that different dynamizations caused different
results. When there is a similitude between the homeopathic solution
and the organism, the vital energy responds, opposing the energy
of the medication to a test of its own energy (Hamly, 1979).
However, when there is no similitude between the homeopathic preparation
and the organism there can be disorder in the metabolic system of
the plant, resulting in several negative growth and development
aspects of the plant.

Effect of Sulphur on the mass of the fresh matter of the
shoot and the root system
Both the mass of the fresh matter of the shoot and the root system
were influenced significantly by the different dynamizations of
Sulphur (Figures 4 and 5).
The application of Sulphur at potency 5 CH resulted in a large
increase in the production of the shoot fresh matter, standing out
among the other studied dynamizations and arriving at values approximately
three times higher than the control and the dynamization 200 CH
(Figure 4). The potencies 12 CH, 30 CH and 1 MCH also increased
the mass of the shoot fresh matter and differed statistically from
the control and the dynamization


The application of Sulphur, independent of the studied dynamization,
resulted in a large increase in the fresh matter mass of the root
system, when compared with the control (Figure 5). The difference
was 10 to 20 times greater in relationship to the control. Cymbopogon
citratus plants treated with Sulphur 30 CH presented a reduction
in the mass of the shoot fresh matter, differing statistically from
the control (Castro, 2002).
Effect of Sulphur on the mass of the shoot dry matter and
the root system
The radish plants that received Sulphur at potencies 5 CH and 12
CH presented a larger increment in the mass of the matter of the
shoot (Figure 6), while the control and the other studied dynamizations
did not differ statistically from each other. The plants treated
with Sulphur 5 CH presented more than twice the mass of the dry
matter of the shoot when compared to the control.

The production of dry matter in the root system was identical to
that observed for the mass of the fresh matter, i.e. all the dynamizations
presented significantly higher dry mass values of the root system
(approximately three times higher) in relationship to the control
(Figure 7). The variable mass of the dry matter should be preferred
to the mass of the fresh matter because it is less subject to environmental
factor fluctuations.

It is interesting to note that, even with the adversity of the
abiotic and biotic factors (the plants grew outdoors), the homeopathic
Sulphur was efficient and improved the general characteristics of
the plants.
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